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Moreover, a growing body of evidence suggests that p53 regulates ferroptosis either by transcriptional or posttranslational mechanisms such as modulating the downstream expression of SLC7A11 , 239 SAT1 (encoding spermidine/spermine N1-acetyltransferase 1), 240 GLS2 (encoding glutaminase 2), 25 and CDKN1A/p21 (encoding cyclin dependent kinase inhibitor 1A), 241 or by directly inhibiting DPP4 activity in colorectal cancer cells

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Cell Culture The human colon cancer cell lines HCT116 and SW620, purchased from American Type Culture Collection (Manassas, VA, USA), were cultured in RPMI medium and DMEM/F12 (HyClone, Logan, UT, USA) respectively, supplemented with 10% foetal bovine serum (HyClone) and 1% penicillin and streptomycin

Energy balance is a critical determinant of cell fate, and insufficient energy availability is a common feature in different cells and tissues in a variety of chronic inflammatory diseases