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After washing with methanol:water (1:1), flavonols were recovered in methanol (5 ml) and individually purified by preparative reversed phase HPLC as described (Dixon et al., 2012), but using a 5100% gradient of acetonitrile

Reference and normal values Limited information from deceased miners shows mercury concentrations in the brain, years after cessation of exposure, of several mg/kg, with still higher values in some parts of the brain

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