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Finally, staining was performed with 3,3-diaminobenzidine (DAB, Sigma, D12384) substrate and counterstained with hematoxylin for nuclear differentiation

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The sound waves are now working to reduce the particle size and force the phospholipids to wrap around the glutathione

Then, again Transketolase enzyme transferring a C2 unit, from Xylulose-5-phosphate to Erythrose-4-phosphate , thus form another molecule of Fructose 6-phosphate and Glyceraldehyde-3-phosphate , both are intermediates of glycolysis