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Reynolds Tobacco Company, Winston-Salem, NC ( TPM preparation TPM from 3R4F reference cigarettes (University of Kentucky) was prepared at Labstat International, Kitchner, Canada as described previously ( Neutrophil isolation and culture Neutrophils from bone marrow were isolated from the tibias and femurs of wild type (WT) mice, purified over a Percoll gradient of 82, 65, and 55%

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The constant exposure to nicotine may contribute to the development of oral problems