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Cells were transfected with 2 g of the indicated pCMV-HA-LMP1 plasmids together with 0.5 g of pRK5-Flag-TRAF6 plasmids or empty vector, 0.05 g of NF-B luciferase reporter 3xB-Luc and 0.2 g of pPGK-Renilla housekeeping control reporter using the PolyFect transfection reagent (Qiagen) 33

Therefore, we treated in vitro activated B6 FoB with various mitochondrial inhibitors and the Gclc inhibitor BSO and measured total immunoglobulins from the supernatants